脐带是哺乳类的连接胎儿和胎盘的管状结构,由两条动脉和一条静脉构成。脐带呈圆柱状结构,内有两条脐动脉及一条脐静脉。脐带内有一条脐静脉,接受从母体来的新鲜血液并流经胎儿全身回到胎儿的动脉系统,最后进入两条脐动脉流入胎盘至母体,这是胎儿血循环的特点之一。胎儿藉以生存的营养物质如电解质、无机盐、激素、微量元素、免疫抗体等,均需经过脐带传送。
脐带也一直是科研人员竞相研究的一块领域,例如脐带间充质干细胞,它联合造血干细胞移植可发挥强大的造血支持能力。科学家发现间充质干细胞具有调节免疫的作用,有着广泛的临床应用前景。它可以改善骨髓造血微环境,促进造血干细胞的植入,显著加快移植后的造血重建,安全且并发症少。同时,适量的间充质干细胞在造血干细胞移植中,可以降低移植物抗宿主病的发生率和严重程度。
山东银丰生物集团就利用上海威九国际浸入式液氮冷冻研磨仪研磨脐带,提取RNA。威九国际便携式液氮冷冻研磨仪功能强大,是世界上技术领先的液氮冷冻研磨设备,全过程超低温运行,是市场上公认的高效的研磨设备:其冷冻研磨方法是一种先进的、高效的、方便的样品处理方法,可以处理常规方法难以处理的样品,如:塑料、橡胶、树脂、皮革、毛发、骨骼、木材、纺织原料等等,被称为研磨的终极手段。液氮冷冻研磨仪应用领域广泛,如生物化学中的RNA/DNA检测、聚合物结构分析、痕迹元素的判断、食品分析、矿物质研究、医疗外科手术的辅助、药品性能的检测、纺织品组成成分的鉴定等等。
冷冻组织研磨仪
实验目的:通过研磨脐带组织,提取RNA
实验材料和器具:
样品:脐带组织
工具:威九国际全自动样品快速研磨仪
耗材:专用研磨罐,液氮、剪刀、镊子等
实验步骤:
1、准备研磨罐和样品;
2、取组织剪成小块放入研磨罐中,将盖子盖紧;
3、将研磨罐置于装有液氮的容器内,液氮容量没过研磨罐冷冻几分钟,取出时戴手套或使用镊子夹取避免液氮冻伤;
4、首先将适配器放入研磨仪中固定,然后在将研磨罐放置适合器内,以保证研磨时的受力平衡,旋紧垂直定位螺旋,将安全锁扣紧。
5、设置参数启动程序结束后即可得到破碎粉末;
6、研磨完毕后请关闭电源,将安全锁归位;
7、完成后续RNA提取步骤。
实验解决的问题:
1、仪器研磨相对手工研磨速度更快,缩短整体实验时间;
2、仪器研磨出料颗粒均匀、细腻,无需过筛,可直接进行下一步提取工作;
3、避免样品污染与浪费
实验过程、结果图示:
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